English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Confocal laser scanning microscopy—a powerful tool in bone research

MPS-Authors
/persons/resource/persons136530

Roschger,  Andreas
Wolfgang Wagermaier, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

External Ressource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Blouin, S., Roschger, A., Varga, F., Misof, B., Spitzer, S., Roschger, P., et al. (2018). Confocal laser scanning microscopy—a powerful tool in bone research. Wiener Medizinische Wochenschrift, 168(11-12), 314-321. doi:10.1007/s10354-018-0639-x.


Cite as: http://hdl.handle.net/21.11116/0000-0001-6501-C
Abstract
The confocal laser scanning microscope (CLSM) enables the collection of images picturing selected planes in depth of thick samples, thus giving 3D information while keeping the sample intact. In this article we give an overview of our CLSM applications in bone research: (i) the characterization of osteoblasts and osteoclasts properties in cell biology, (ii) the visualization of the three dimensional (3D) osteocyte lacunar canalicular network in undemineralized plastic-embedded bone samples, (iii) the observation of tetracycline labels in bone biopsy samples from patients in combination with information on the mineralization density from quantitative backscatter electron imaging, which enables the time course of mineral accumulation in newly formed bone to be followed, (iv) the precise measurement of the thickness of thin ground bone sections, a prerequisite for the mapping of local mechanical properties by scanning acoustic microscopy.