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Preprint

Tissue embedding in a silica hydrogel for functional investigations

MPS-Authors
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Miazzi,  Fabio
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons3955

Kaltofen,  Sabine
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons222618

Bello,  Jan
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons3909

Hansson,  Bill S.
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Wicher,  Dieter
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

External Resource

http://dx.doi.org/10.1101/357962
(Preprint)

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Fulltext (public)

HAN311.pdf
(Preprint), 760KB

Supplementary Material (public)

HAN311s1.pdf
(Supplementary material), 826KB

HAN311s2.mov
(Supplementary material), 22MB

Citation

Miazzi, F., Kaltofen, S., Bello, J., Hansson, B. S., & Wicher, D. (2018). Tissue embedding in a silica hydrogel for functional investigations. bioRxiv: the preprint server for biology. doi:10.1101/357962.


Cite as: https://hdl.handle.net/21.11116/0000-0001-976D-B
Abstract
Embedding procedures can be challenging for delicate specimens. We present a method based on a sodium metasilicate (waterglass) gel to embed tissue samples for acute physiological studies. We show that the application of such a colloidal gel has minimal effects on the properties of buffered solutions and cell activity, allowing functional investigations on sensitive cells such as ciliated insect olfactory neurons.