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Surface properties determining passage rates of proteins through nuclear pores.

MPG-Autoren
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Frey,  S.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

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Rees,  R.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

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Schünemann,  J.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

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Ng,  S. C.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

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Fünfgeld,  K.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

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Huyton,  T.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

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Görlich,  D.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

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Zitation

Frey, S., Rees, R., Schünemann, J., Ng, S. C., Fünfgeld, K., Huyton, T., et al. (2018). Surface properties determining passage rates of proteins through nuclear pores. Cell, 174(1), 202-217. doi:10.1016/j.cell.2018.05.045.


Zitierlink: https://hdl.handle.net/21.11116/0000-0001-9899-7
Zusammenfassung
Nuclear pore complexes (NPCs) conduct nucleocytoplasmic transport through an FG domain-controlled barrier. We now explore how surface-features of a mobile species determine its NPC passage rate. Negative charges and lysines impede passage. Hydrophobic residues, certain polar residues (Cys, His), and, surprisingly, charged arginines have striking translocation-promoting effects. Favorable cation-π interactions between arginines and FG-phenylalanines may explain this apparent paradox. Application of these principles to redesign the surface of GFP resulted in variants that show a wide span of transit rates, ranging from 35-fold slower than wild-type to ∼500 times faster, with the latter outpacing even naturally occurring nuclear transport receptors (NTRs). The structure of a fast and particularly FG-specific GFPNTR variant illustrates how NTRs can expose multiple regions for binding hydrophobic FG motifs while evading non-specific aggregation. Finally, we document that even for NTR-mediated transport, the surface-properties of the "passively carried" cargo can strikingly affect the translocation rate.