Reduced dyes enhance single-molecule localization density for live 
superresolution imaging.

Carlini, L.; Benke, A.; Reymond, L.; Lukinavicius, G.; Manley, S.

doi:10.1002/cphc.201301004

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Reduced dyes enhance single-molecule localization density for live superresolution imaging.

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Lukinavicius, G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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Citation

Carlini, L., Benke, A., Reymond, L., Lukinavicius, G., & Manley, S. (2014). Reduced dyes enhance single-molecule localization density for live superresolution imaging. ChemPhysChem, 15(4), 750-755. doi:10.1002/cphc.201301004.

Cite as: https://hdl.handle.net/21.11116/0000-0001-A61C-5

Abstract

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can result in up to ten-fold higher densities of single molecule localizations, and more photons per localization as compared with unmodified dyes. These two parameters directly impact the achievable resolution, and we see a significant improvement in the quality of live-cell point-localization super-resolution images taken with reduced dyes. These improvements carry over to increase the density of trajectories for single-molecule tracking experiments.