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Journal Article

Holographic imaging of macromolecules


Grunze,  M.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Gölzhäuser, A., Völkel, B., Jäger, B., Zharnikov, M., Kreuzer, H., & Grunze, M. (1998). Holographic imaging of macromolecules. Journal of Vacuum Science and Technology A: Vacuum, Surfaces, and Films, 16(5), 3025-3028. doi:10.1116/1.581454.

Cite as: https://hdl.handle.net/21.11116/0000-0001-B69B-3
The low energy electron point source microscope (kendroscope) is used to acquire in-line holograms of rodlike macromolecules of phthalocyaninato polysiloxane (PcPS). The molecules were placed on a new class of substrates, thin microstructured silicon membranes that have slits about 100 nm wide which can be “bridged” by the molecular chains. The resulting in-line holograms of PcPS were recorded digitally and reconstructed numerically via a Kirchoff–Helmholtz transform. The mechanisms of image formation and uncertainties relating to the interpretation of the holograms are discussed.