Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Methyl angolensate changes in Khaya ivorensis after fungal infection

MPG-Autoren
Es sind keine MPG-Autoren in der Publikation vorhanden
Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Zitation

Reche, K. V. G., de Souza, G. D., Trapp, M. A., Rodrigues-Filho, E., Silva, S. C., Fernandes, J. B., et al. (2009). Methyl angolensate changes in Khaya ivorensis after fungal infection. Phytochemistry, 70(17-18), 2027-2033. doi:10.1016/j.phytochem.2009.08.013.


Zitierlink: http://hdl.handle.net/21.11116/0000-0001-BFCE-1
Zusammenfassung
Khaya ivorensis with and without symptoms of stem and branch cankers, caused by Botryosphaeria rhodina were examined in order to determine whether the secondary metabolites in this plant were associated with a chemical defense response. This study provides evidence that the limonoid methyl angolensate (MA) is present at higher concentrations in K. ivorensis with symptoms of stem cankers rather than in the plants without symptoms. A rapid, sensitive and selective HPLC–ESI-MS/MS method (using selected reaction monitoring – SRM – mode) was developed for quantification of MA in all aerials parts of such plants, with a good linearity over a range of 0.1–20.0 g/kg, with r2 > 0.996 ± 6.1%. The limits of detection (LOD) and quantification (LOQ) were less than 0.03 g/kg and 0.08 g/kg, respectively. Relative Standard Deviations (RSDs) ranged from 1.7% to 19.2% for all matrices. While the MA concentration did not change in the stem bark, its amounts increased nearly fourfold in stems and by 20% in leaves, when plants with symptoms were compared with those without symptoms. These data suggest that MA plays a role in plant–pathogen interactions, probably as a phytoanticipin.