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Efficiency of tissue homogenization via picosecond-infrared laser (PIRL) and classical homogenization as sample preparation step for proteomics

MPS-Authors
/persons/resource/persons136030

Hansen,  N.-O.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons136024

Miller,  R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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https://www.dgms2016.uni-hamburg.de/program/dgms2016-abstracts-poster.pdf
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Citation

Nimer, R., Kwiatkowski, M., Hansen, N.-O., Miller, R. J. D., & Schlüter, H. (2016). Efficiency of tissue homogenization via picosecond-infrared laser (PIRL) and classical homogenization as sample preparation step for proteomics. Poster presented at 49. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie (DGMS), Hamburg.


Cite as: https://hdl.handle.net/21.11116/0000-0001-DE3A-5
Abstract
Introduction: A novel method for ultrafast and soft cold vaporization of tissue via desorption by impulsiv e vibrational excitation (DIVE) using a picosecond - infrared laser (PIRL) has been developed by Dwayne Miller and his group [1]. By irradiating tissue with PIRL proteins are transferred from the tissue into the gas phase within at aerosol plume without chan ging the chemical formula of protein. The chemical composition of the protein is maintained during tissue ablation via PIRL. In this study, the efficiency of tissue homogenization performed with PIRL and with classical homogenization was investigated using two - dimensional electrophoresis (2DE) followed by mass spectrometric analysis (MS) of the tryptic peptides of the separated proteins from the 2DE gel.