Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
  Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben
Poster

Efficiency of tissue homogenization via picosecond-infrared laser (PIRL) and classical homogenization as sample preparation step for proteomics

MPG-Autoren
/persons/resource/persons136030

Hansen,  N.-O.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

/persons/resource/persons136024

Miller,  R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Externe Ressourcen

https://www.dgms2016.uni-hamburg.de/program/dgms2016-abstracts-poster.pdf
(beliebiger Volltext)

Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)

dgms2016-abstracts-poster.pdf
(beliebiger Volltext), 2MB

Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Nimer, R., Kwiatkowski, M., Hansen, N.-O., Miller, R. J. D., & Schlüter, H. (2016). Efficiency of tissue homogenization via picosecond-infrared laser (PIRL) and classical homogenization as sample preparation step for proteomics. Poster presented at 49. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie (DGMS), Hamburg.


Zitierlink: https://hdl.handle.net/21.11116/0000-0001-DE3A-5
Zusammenfassung
Introduction: A novel method for ultrafast and soft cold vaporization of tissue via desorption by impulsiv e vibrational excitation (DIVE) using a picosecond - infrared laser (PIRL) has been developed by Dwayne Miller and his group [1]. By irradiating tissue with PIRL proteins are transferred from the tissue into the gas phase within at aerosol plume without chan ging the chemical formula of protein. The chemical composition of the protein is maintained during tissue ablation via PIRL. In this study, the efficiency of tissue homogenization performed with PIRL and with classical homogenization was investigated using two - dimensional electrophoresis (2DE) followed by mass spectrometric analysis (MS) of the tryptic peptides of the separated proteins from the 2DE gel.