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Targeted photoswitchable probe for nanoscopy of biological structures.

MPG-Autoren
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Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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Zitation

Dellagiacoma , C., Lukinavicius, G., Boccio, N., Banala, S., Geissbühler, S., Märki, I., et al. (2010). Targeted photoswitchable probe for nanoscopy of biological structures. ChemBioChem, 11(10), 1361-1363. doi:10.1002/cbic.201000189.


Zitierlink: http://hdl.handle.net/21.11116/0000-0001-DED7-3
Zusammenfassung
SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with ∼25 nm resolution. The described probe in combination with the versatile SNAP‐tag labeling opens new possibilities for imaging biological structures at the nanoscale.