English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Targeted photoswitchable probe for nanoscopy of biological structures.

MPS-Authors
/persons/resource/persons203920

Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)

2628651_Suppl.pdf
(Supplementary material), 2MB

Citation

Dellagiacoma , C., Lukinavicius, G., Boccio, N., Banala, S., Geissbühler, S., Märki, I., et al. (2010). Targeted photoswitchable probe for nanoscopy of biological structures. ChemBioChem, 11(10), 1361-1363. doi:10.1002/cbic.201000189.


Cite as: https://hdl.handle.net/21.11116/0000-0001-DED7-3
Abstract
SNAP‐tag: We introduce a photoswitchable O6‐benzylguanine derivative and demonstrate its use for super‐resolution microscopy of SNAP‐tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP‐tagged microtubule structures with ∼25 nm resolution. The described probe in combination with the versatile SNAP‐tag labeling opens new possibilities for imaging biological structures at the nanoscale.