English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Cytosine-5-methyltransferases add aldehydes to DNA.

MPS-Authors
/persons/resource/persons203920

Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)

2629768_Suppl.172
(Supplementary material), 184KB

Citation

Liutkevičiūtė, Z., Lukinavicius, G., Masevičius, V., Daujotytė, D., & Klimašauskas, S. (2009). Cytosine-5-methyltransferases add aldehydes to DNA. Nature Chemical Biology, 5(6), 400-402. doi:10.1038/nchembio.172.


Cite as: http://hdl.handle.net/21.11116/0000-0001-DFCF-C
Abstract
Targeted methylation of cytosine residues by S-adenosylmethionine–dependent DNA methyltransferases modulates gene expression in vertebrates. Here we show that cytosine-5-methyltransferases catalyze reversible covalent addition of exogenous aliphatic aldehydes to their target residues in DNA, thus yielding corresponding 5-α-hydroxyalkylcytosines. Such atypical enzymatic reactions with non-cofactor-like substrates open new ways for sequence-specific derivatization of DNA and demonstrate enzymatic exchange of 5-hydroxymethyl groups on cytosine in support of an oxidative mechanism of DNA demethylation.