Cytosine-5-methyltransferases add aldehydes to DNA.

Liutkevičiūtė, Z.; Lukinavicius, G.; Masevičius, V.; Daujotytė, D.; Klimašauskas, S.

doi:10.1038/nchembio.172

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Cytosine-5-methyltransferases add aldehydes to DNA.

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Lukinavicius, G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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2629768_Suppl.172
(Supplementary material), 184KB

Citation

Liutkevičiūtė, Z., Lukinavicius, G., Masevičius, V., Daujotytė, D., & Klimašauskas, S. (2009). Cytosine-5-methyltransferases add aldehydes to DNA. Nature Chemical Biology, 5(6), 400-402. doi:10.1038/nchembio.172.

Cite as: https://hdl.handle.net/21.11116/0000-0001-DFCF-C

Abstract

Targeted methylation of cytosine residues by S-adenosylmethionine–dependent DNA methyltransferases modulates gene expression in vertebrates. Here we show that cytosine-5-methyltransferases catalyze reversible covalent addition of exogenous aliphatic aldehydes to their target residues in DNA, thus yielding corresponding 5-α-hydroxyalkylcytosines. Such atypical enzymatic reactions with non-cofactor-like substrates open new ways for sequence-specific derivatization of DNA and demonstrate enzymatic exchange of 5-hydroxymethyl groups on cytosine in support of an oxidative mechanism of DNA demethylation.