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Journal Article

Targeted labeling of DNA by methyltransferase-directed transfer of activated groups (mTAG).

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Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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Citation

Lukinavicius, G., Lapienė, V., Staševskij, Z., Dalhoff, C., Weinhold, E., & Klimašauskas, S. (2007). Targeted labeling of DNA by methyltransferase-directed transfer of activated groups (mTAG). Journal of the American Chemical Society, 129(10), 2758-2759. doi:10.1021/ja0691876.


Cite as: http://hdl.handle.net/21.11116/0000-0001-DFEE-9
Abstract
Methyltransferases catalyze highly specific transfers of methyl groups from the ubiquitous cofactor S-adenosyl-l-methionine (AdoMet) to various biopolymers like DNA, RNA, and proteins. Here we describe the first synthetic analogue of AdoMet with an activated side chain carrying a primary amino group that permits efficient methyltransferase-directed functionalization of DNA and subsequent amine-specific chemoligations with various reporter groups. The demonstrated two-step sequence-specific labeling of natural DNA offers a facile way to query the methylation status of the target sites and envisions numerous applications in functional studies and medical diagnostics.