TCRs are randomly distributed on the plasma membrane of resting 
antigen-experienced T cells.

Rossboth, B.; Arnold, A. M.; Ta, H.; Platzer, R.; Kellner, F.; Huppa, J. B.; Brameshuber, M.; Baumgart, F.; Schütz, G. J.

doi:10.1038/s41590-018-0162-7

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Zeitschriftenartikel

TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells.

MPG-Autoren

/persons/resource/persons79571

Ta, H.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

Externe Ressourcen

Es sind keine externen Ressourcen hinterlegt

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Es sind keine frei zugänglichen Volltexte in PuRe verfügbar

Ergänzendes Material (frei zugänglich)

2630078_Suppl_1.pdf
(Ergänzendes Material), 5MB

2630078_Suppl_2.pdf
(Ergänzendes Material), 81KB

Zitation

Rossboth, B., Arnold, A. M., Ta, H., Platzer, R., Kellner, F., Huppa, J. B., et al. (2018). TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells. Nature Immunology, 19(8), 821-827. doi:10.1038/s41590-018-0162-7.

Zitierlink: https://hdl.handle.net/21.11116/0000-0001-E238-1

Zusammenfassung

The main function of T cells is to identify harmful antigens as quickly and precisely as possible. Super-resolution microscopy data have indicated that global clustering of T cell antigen receptors (TCRs) occurs before T cell activation. Such pre-activation clustering has been interpreted as representing a potential regulatory mechanism that fine tunes the T cell response. We found here that apparent TCR nanoclustering could be attributed to overcounting artifacts inherent to single-molecule-localization microscopy. Using complementary super-resolution approaches and statistical image analysis, we found no indication of global nanoclustering of TCRs on antigen-experienced CD4(+) T cells under non-activating conditions. We also used extensive simulations of super-resolution images to provide quantitative limits for the degree of randomness of the TCR distribution. Together our results suggest that the distribution of TCRs on the plasma membrane is optimized for fast recognition of antigen in the first phase of T cell activation.