Datensatz

Zusammenfassung

Polysaccharides from plant biomass are explored
extensively as renewable resources for the production of materials
and fuels. However, the heterogeneous nature of non-cellulosic
polysaccharides such as arabinoxylan makes it difficult to correlate
molecular structure with macroscopic properties. To study the impact
of specific structural features of the polysaccharides on e.g.
crystallinity or affinity to other cell wall components, collections of
polysaccharides with defined repeating units are required. Herein, a
chemo-enzymatic approach towards artificial arabinoxylan
polysaccharides with systematically altered branching patterns is
described. The polysaccharides were obtained by glycosynthasecatalyzed
polymerization of glycosyl fluorides derived from
arabinoxylan oligosaccharides that were procured either chemically,
chemo-enzymatically, or from a commercial source. X-ray diffraction
and adsorption experiments on cellulosic surfaces revealed that the
physico-chemical properties of the synthetic polysaccharides
strongly depend on the specific nature of their substitution patterns.
The presented strategy of combining sophisticated carbohydrate
synthesis with glycosynthase technology offers access to artificial
polysaccharides for structure-property relationship studies that are
not accessible by other means.