English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1.

MPS-Authors
/persons/resource/persons135076

Maracci,  C.
Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15723

Rodnina,  M. V.
Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)

2630294.pdf
(Publisher version), 7MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Graf, M., Huter, P., Maracci, C., Peterek, M., Rodnina, M. V., & Wilson, D. N. (2018). Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1. Nature Communications, 9: 3053. doi:10.1038/s41467-018-05465-1.


Cite as: http://hdl.handle.net/21.11116/0000-0001-E52E-A
Abstract
During translation termination in bacteria, the release factors RF1 and RF2 are recycled from the ribosome by RF3. While high-resolution structures of the individual termination factors on the ribosome exist, direct structural insight into how RF3 mediates dissociation of the decoding RFs has been lacking. Here we have used the Apidaecin 137 peptide to trap RF1 together with RF3 on the ribosome and visualize an ensemble of termination intermediates using cryo-electron microscopy. Binding of RF3 to the ribosome induces small subunit (SSU) rotation and swivelling of the head, yielding intermediate states with shifted P-site tRNAs and RF1 conformations. RF3 does not directly eject RF1 from the ribosome, but rather induces full rotation of the SSU that indirectly dislodges RF1 from its binding site. SSU rotation is coupled to the accommodation of the GTPase domain of RF3 on the large subunit (LSU), thereby promoting GTP hydrolysis and dissociation of RF3 from the ribosome.