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Journal Article

Organization of focal adhesion plaques is disrupted by action of the HIV-1 protease

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Shoeman,  Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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https://onlinelibrary.wiley.com/doi/pdf/10.1006/cbir.2002.0895
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https://doi.org/10.1006/cbir.2002.0895
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Citation

Shoeman, R. L., Hartig, R., Hauses, C., & Traub, P. (2002). Organization of focal adhesion plaques is disrupted by action of the HIV-1 protease. Cell Biology International, 26(6), 529-539. doi:10.1006/cbir.2002.0895.


Cite as: https://hdl.handle.net/21.11116/0000-0001-EF81-0
Abstract
Focal adhesion plaques were severely affected in human embryonic fibroblasts permeabilized with digitonin and incubated in buffer containing the human immunodeficiency virus type 1 protease (HIV-1 PR). A mutant HIV-1 PR (3271 HIV-1 PR) had no effect on focal adhesion plaques. Similar effects were seen with cells microinjected with either HIV-1 PR or 3271 HIV-1 PR. Immunoblots of the human embryonic fibroblasts demonstrated that a number of focal adhesion plaque proteins were specifically cleaved by HIV-1 PR. These included fimbrin, focal adhesion plaque kinase (FAK), talin, and, to a lesser extent, filamin, spectrin and fibronectin. Proteins detected by antibodies to beta 4 integrin and alpha 3 integrin were also cleaved by the HIV-1 PR. Control experiments demonstrated that the effect and protein cleavages described are due to action of the HIV-1 PR and not to the action of endogenous host cell proteases.