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Photothermal Control of Heat-Shock Protein Expression at the Single Cell Level

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Verghese,  Jacob
Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Polleux,  Julien
Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Robert, H. M. L., Savatier, J., Vial, S., Verghese, J., Wattellier, B., Rigneault, H., et al. (2018). Photothermal Control of Heat-Shock Protein Expression at the Single Cell Level. Small, 14(32): 1801910. doi:10.1002/smll.201801910.


Cite as: http://hdl.handle.net/21.11116/0000-0001-F7AA-9
Abstract
Laser heating of individual cells in culture recently led to seminal studies in cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase in such experiments remains a challenge. Here, the laser-induced dynamical control of the heat-shock response is demonstrated at the single cell level, enabled by the use of light-absorbing gold nanoparticles as nanosources of heat and a temperature mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it is label-free, this approach does not suffer from artifacts inherent to previously reported fluorescence-based temperature-mapping techniques and enables the use of any standard fluorescent labels to monitor in parallel the cell's response.