Molecular and structural architecture of polyQ aggregates in yeast

Gruber, Anselm; Hornburg, Daniel; Antonin, Matthias; Krahmer, Natalie; Collado, Javier; Schaffer, Miroslava; Zubaite, Greta; Luechtenborg, Christian; Sachsenheimer, Timo; Bruegger, Britta; Mann, Matthias; Baumeister, Wolfgang; Hartl, F. Ulrich; Hipp, Mark S.; Fernandez-Busnadiego, Ruben

doi:10.1073/pnas.1717978115

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学術論文

Molecular and structural architecture of polyQ aggregates in yeast

MPS-Authors

/persons/resource/persons229234

Gruber, Anselm
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons135434

Hornburg, Daniel
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons139964

Antonin, Matthias
Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78265

Krahmer, Natalie
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons208859

Collado, Javier
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons86649

Schaffer, Miroslava
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229236

Zubaite, Greta
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78356

Mann, Matthias
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77721

Baumeister, Wolfgang
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78072

Hartl, F. Ulrich
Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78111

Hipp, Mark S.
Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77952

Fernandez-Busnadiego, Ruben
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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引用

Gruber, A., Hornburg, D., Antonin, M., Krahmer, N., Collado, J., Schaffer, M., Zubaite, G., Luechtenborg, C., Sachsenheimer, T., Bruegger, B., Mann, M., Baumeister, W., Hartl, F. U., Hipp, M. S., & Fernandez-Busnadiego, R. (2018). Molecular and structural architecture of polyQ aggregates in yeast. Proceedings of the National Academy of Sciences of the United States of America, 115(15), E3446-E3453. doi:10.1073/pnas.1717978115.

引用: https://hdl.handle.net/21.11116/0000-0002-C782-A

要旨

Huntington's disease is caused by the expansion of a polyglutamine (polyQ) tract in the N-terminal exon of huntingtin (HttEx1), but the cellular mechanisms leading to neurodegeneration remain poorly understood. Here we present in situ structural studies by cryoelectron tomography of an established yeast model system of polyQ toxicity. We find that expression of polyQ-expanded HttEx1 results in the formation of unstructured inclusion bodies and in some cases fibrillar aggregates. This contrasts with recent findings in mammalian cells, where polyQ inclusions were exclusively fibrillar. In yeast, polyQ toxicity correlates with alterations in mitochondrial and lipid droplet morphology, which do not arise from physical interactions with inclusions or fibrils. Quantitative proteomic analysis shows that polyQ aggregates sequester numerous cellular proteins and cause a major change in proteome composition, most significantly in proteins related to energy metabolism. Thus, our data point to a multifaceted toxic gain-of-function of polyQ aggregates, driven by sequestration of endogenous proteins and mitochondrial and lipid droplet dysfunction.