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Single-Molecule Sequencing Reveals the Chromosome-Scale Genomic Architecture of the Nematode Model Organism Pristionchus pacificus

MPG-Autoren

Roedelsperger,  Christian
Max Planck Institute for Developmental Biology, Max Planck Society;

Meyer,  Jan M.
Max Planck Institute for Developmental Biology, Max Planck Society;

Prabh,  Neel
Max Planck Institute for Developmental Biology, Max Planck Society;

Lanz,  Christa
Max Planck Institute for Developmental Biology, Max Planck Society;

Bemm,  Felix
Max Planck Institute for Developmental Biology, Max Planck Society;

Sommer,  Ralf J.
Max Planck Institute for Developmental Biology, Max Planck Society;

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Zitation

Roedelsperger, C., Meyer, J. M., Prabh, N., Lanz, C., Bemm, F., & Sommer, R. J. (2017). Single-Molecule Sequencing Reveals the Chromosome-Scale Genomic Architecture of the Nematode Model Organism Pristionchus pacificus. CELL REPORTS, 21(3), 834-844. doi:10.1016/j.celrep.2017.09.077.


Zitierlink: http://hdl.handle.net/21.11116/0000-0002-03E5-8
Zusammenfassung
The nematode Pristionchus pacificus is an established model for integrative evolutionary biology and comparative studies with Caenorhabditis elegans. While an existing genome draft facilitated the identification of several genes controlling various developmental processes, its high degree of fragmentation complicated virtually all genomic analyses. Here, we present a de novo genome assembly fromsingle-molecule, long-read sequencing data consisting of 135 P. pacificus contigs. When combined with a genetic linkage map, 99% of the assembly could be ordered and oriented into six chromosomes. This allowed us to robustly characterize chromosomal patterns of gene density, repeat content, nucleotide diversity, linkage disequilibrium, and macrosynteny in P. pacificus. Despite widespread conservation of synteny between P. pacificus and C. elegans, we identified one major translocation from an autosome to the sex chromosome in the lineage leading to C. elegans. This highlights the potential of the chromosome-scale assembly for future genomic studies of P. pacificus.