English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Contribution of major FLM isoforms to temperature-dependent flowering in Arabidopsis thaliana

MPS-Authors

Capovilla,  Giovanna
Max Planck Institute for Developmental Biology, Max Planck Society;

Symeonidi,  Efthymia
Max Planck Institute for Developmental Biology, Max Planck Society;

Wu,  Rui
Max Planck Institute for Developmental Biology, Max Planck Society;

Schmid,  Markus
Max Planck Institute for Developmental Biology, Max Planck Society;
External Organizations;

External Ressource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Capovilla, G., Symeonidi, E., Wu, R., & Schmid, M. (2017). Contribution of major FLM isoforms to temperature-dependent flowering in Arabidopsis thaliana. Journal of Experimental Botany, 68(18), 5117-5127. doi:10.1093/jxb/erx328.


Cite as: http://hdl.handle.net/21.11116/0000-0002-047F-C
Abstract
FLOWERING LOCUS M (FLM), a component of the thermosensory flowering time pathway in Arabidopsis thaliana, is regulated by temperature-dependent alternative splicing (AS). The main splicing variant, FLM-beta, is a well-documented floral repressor that is down-regulated in response to increasing ambient growth temperature. Two hypotheses have been formulated to explain how flowering time is modulated by AS of FLM. In the first model a second splice variant, FLM-delta, acts as a dominant negative isoform that competes with FLM-beta at elevated ambient temperatures, thereby indirectly promoting flowering. Alternatively, it has been suggested that the induction of flowering at elevated temperatures is caused only by reduced FLM-beta expression. To better understand the role of the two FLM splice forms, we employed CRISPR/Cas9 technology to specifically delete the exons that characterize each splice variant. Lines that produced repressive FLM-beta but were incapable of producing FLM-delta were late flowering. In contrast, FLM-beta knockout lines that still produced FLM-delta flowered early, but not earlier than the flm-3 loss of function mutant, as would be expected if FLM-delta had a dominant-negative effect on flowering. Our data support the role of FLM-beta as a flower repressor and provide evidence that a contribution of FLM-delta to the regulation of flowering time in wild-type A. thaliana seems unlikely.