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Journal Article

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

MPS-Authors

Lanz,  Christa
Max Planck Institute for Developmental Biology, Max Planck Society;

Weigel,  Detlef
Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017). Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports, 7: 482. doi:10.1038/s41598-017-00578-x.


Cite as: http://hdl.handle.net/21.11116/0000-0002-04E9-3
Abstract
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non- transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/ Cas9 technology. We used wholegenome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/ Cas9 being a highly precise tool, as we did not detect off- target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.