Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

A cdk1 gradient guides surface contraction waves in oocytes.

MPG-Autoren
/persons/resource/persons225731

Lenart,  P.
Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)

2640170.pdf
(Verlagsversion), 6MB

Ergänzendes Material (frei zugänglich)

2640170_Suppl_1.pdf
(Ergänzendes Material), 286KB

2640170_Suppl_2.pdf
(Ergänzendes Material), 2MB

Zitation

Bischof, J., Brand, C. A., Somogyi, K., Májer, I., Thome, S., Mori, M., et al. (2017). A cdk1 gradient guides surface contraction waves in oocytes. Nature Communications, 8: 849. doi:10.1038%2Fs41467-017-00979-6.


Zitierlink: http://hdl.handle.net/21.11116/0000-0002-0CB9-1
Zusammenfassung
Surface contraction waves (SCWs) in oocytes and embryos lead to large-scale shape changes coupled to cell cycle transitions and are spatially coordinated with the cell axis. Here, we show that SCWs in the starfish oocyte are generated by a traveling band of myosin II-driven cortical contractility. At the front of the band, contractility is activated by removal of cdk1 inhibition of the RhoA/RhoA kinase/myosin II signaling module, while at the rear, contractility is switched off by negative feedback originating downstream of RhoA kinase. The SCW’s directionality and speed are controlled by a spatiotemporal gradient of cdk1-cyclinB. This gradient is formed by the release of cdk1-cyclinB from the asymmetrically located nucleus, and progressive degradation of cyclinB. By combining quantitative imaging, biochemical and mechanical perturbations with mathematical modeling, we demonstrate that the SCWs result from the spatiotemporal integration of two conserved regulatory modules, cdk1-cyclinB for cell cycle regulation and RhoA/Rok/NMYII for actomyosin contractility.