Datensatz

Zusammenfassung

Jasmonates are important phytohormones involved in both plant developmental processes as well as defense reactions. Many
JA-mediated plant defense responses have been studied in model plants using mutants of the jasmonate signaling pathway.
However, in plant species where JA-signaling mutants are not accessible, the availability of a tool targeting JA signaling is
crucial to investigate jasmonate-dependent processes. Neomycin is a poly-cationic aminoglycoside antibiotic that blocks the
release of Ca2+
from internal stores. We examined the inhibitory activities of neomycin on different jasmonate-inducible
responses in eight different plant species: Intracellular calcium measurements in Nicotiana tabacum cell culture, Sporamin
gene induction in Ipomoea batatas, PDF2.2 gene expression in Triticum aestivum, Nepenthesin protease activity measurement
in Nepenthes alata, extrafloral nectar production in Phaseolus lunatus, nectary formation in Populus trichocarpa, terpene
accumulation in Picea abies, and secondary metabolite generation in Nicotiana attenuata. We are able to show that neomycin,
an easily manageable and commercially available compound, inhibits JA-mediated responses across the plant kingdom.