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The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

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Schulz,  E.-C.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Mehrabi,  P.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Müller-Werkmeister,  H.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Physical Chemistry, Institute of Chemistry, University of Potsdam;

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Tellkamp,  F.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Jha,  A.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Stuart,  W.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Miller,  R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Departments of Chemistry and Physics, University of Toronto;
Department of Physics, Centre for Ultrafast Imaging, University of Hamburg;

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Citation

Schulz, E.-C., Mehrabi, P., Müller-Werkmeister, H., Tellkamp, F., Jha, A., Stuart, W., et al. (2018). The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. Nature methods, 15(11), 901-904. doi:10.1038/s41592-018-0180-2.


Cite as: http://hdl.handle.net/21.11116/0000-0002-6B31-F
Abstract
We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.