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Journal Article

Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing

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Glocke,  Isabelle
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Ayinuer-Petri,  Aximu
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Meyer,  Matthias
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Citation

Rohland, N., Glocke, I., Ayinuer-Petri, A., & Meyer, M. (2018). Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing. Nature Protocols, 13, 2447-2461. doi:10.1038/s41596-018-0050-5.


Cite as: https://hdl.handle.net/21.11116/0000-0002-71AC-D
Abstract
DNA preserved in ancient bones, teeth and sediments is typically highly fragmented and present only in minute amounts.
Here, we provide a highly versatile silica-based DNA extraction protocol that enables the retrieval of short (≥35 bp) or
even ultrashort (≥25 bp) DNA fragments from such material with minimal carryover of substances that inhibit library
preparation for high-throughput sequencing. DNA extraction can be performed with either silica spin columns, which offer
the most convenient choice for manual DNA extraction, or silica-coated magnetic particles. The latter allow a substantial
cost reduction as well as automation on liquid-handling systems. This protocol update replaces a now-outdated version
that was published 11 years ago, before high-throughput sequencing technologies became widely available. It has been
thoroughly optimized to provide the highest DNA yields from highly degraded samples, as well as fast and easy handling, requiring not more than ~15 min of hands-on time per sample.
This protocol is an update to: Nat. Protoc. 2, 1756–1762 (2007): https://doi.org/10.1038/nprot.2007.247