English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Near-infrared STED nanoscopy with an engineered bacterial phytochrome.

MPS-Authors
/persons/resource/persons208316

Kamper,  M.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons79571

Ta,  H.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons128160

Jensen,  N. A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15269

Jakobs,  S.
Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society;

Locator
There are no locators available
Fulltext (public)

3008863.pdf
(Publisher version), 5MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Kamper, M., Ta, H., Jensen, N. A., Hell, S. W., & Jakobs, S. (2018). Near-infrared STED nanoscopy with an engineered bacterial phytochrome. Nature Communications, 9: 4762. doi:10.1038/s41467-018-07246-2.


Cite as: http://hdl.handle.net/21.11116/0000-0002-7E20-D
Abstract
The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.