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Resolution and Enhancement in Nanoantenna-Based Fluorescence Microscopy

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Eghlidi, H., Lee, K. G., Chen, X.-W., Götzinger, S., & Sandoghdar, V. (2009). Resolution and Enhancement in Nanoantenna-Based Fluorescence Microscopy. Nano Letters, 9, 4007-4011. doi:10.1021/nl902183y.


Cite as: http://hdl.handle.net/21.11116/0000-0002-9A8B-4
Abstract
Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the diameter of the gold nanoparticle. We examine the interplay between fluorescence enhancement and spatial resolution and discuss the requirements for deciphering single molecules in a dense sample. Resolutions better than 20 nm and fluorescence enhancement up to 30 times are demonstrated experimentally. By accounting for the tip shaft and the sample interface in finite-difference time-domain calculations, we explain why the measured fluorescence enhancements are higher in the presence of an interface than the values predicted for a homogeneous environment.