Unlocking the diversity of alkaloids in Catharanthus roseus: nuclear 
localization suggests metabolic channeling in secondary metabolism

Stavrinides, Anna; Tatsis, Evangelos C.; Foureau, Emilien; Caputi, Lorenzo; Kellner, Franziska; Courdavault, Vincent; O'Connor, Sarah E.

doi:10.1016/j.chembiol.2015.02.006

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Unlocking the diversity of alkaloids in Catharanthus roseus: nuclear localization suggests metabolic channeling in secondary metabolism

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372254/
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Stavrinides, A., Tatsis, E. C., Foureau, E., Caputi, L., Kellner, F., Courdavault, V., et al. (2015). Unlocking the diversity of alkaloids in Catharanthus roseus: nuclear localization suggests metabolic channeling in secondary metabolism. Chemistry & Biology, 22(3), 336-341. doi:10.1016/j.chembiol.2015.02.006.

Cite as: https://hdl.handle.net/21.11116/0000-0002-B6F4-D

Abstract

The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway.