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Journal Article

Structure of actin-containing filaments from two types of non-muscle cells


Mandelkow,  Eckhard
Arbeitsgruppe Zytoskelett, Max Planck Institute for Medical Research, Max Planck Society;

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DeRosier, D., Mandelkow, E., Silliman, A., Tilney, L., & Kane, R. (1977). Structure of actin-containing filaments from two types of non-muscle cells. Journal of Molecular Biology (London), 113(4), 679-695. doi:10.1016/0022-2836(77)90230-3.

Cite as: http://hdl.handle.net/21.11116/0000-0002-ADCA-8
Bundles of actin-containing filaments from the acrosomal process of horseshoe crab sperm and from sea urchin egg contain a second protein having a molecular weight of about 55,000. Electron micrographs of these filamentous bundles show features reminiscent of paracrystalline arrays of actin except that bundles from the sea urchin egg have distinctive transverse bands every 110 Å. From optical diffraction patterns of the micrographs, we deduced very similar models for both structures. The models consist of hexagonal arrays of actin filaments cross-linked by the second protein. The pattern of transverse bands in bundles derived from the sea urchin eggs is accounted for by postulating that the second protein is bonded to actin only at positions where cross-linking can occur, rather than being bonded to every actin. The helical symmetry of the actin requires that the bonding contacts involved in the cross-linking be slightly different at different positions along the length of the bundle. The technique of image reconstruction was used to obtain a three-dimensional map of the bundles from the acrosomal process.