Interaction of DNA with DNA‐binding proteins: the characterization of protein 
HD from Escherichia coli and its nucleic acid complexes

Berthold, Verena; Geider, Klaus

doi:10.1111/j.1432-1033.1976.tb11132.x

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Interaction of DNA with DNA‐binding proteins: the characterization of protein HD from Escherichia coli and its nucleic acid complexes

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Geider, Klaus
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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https://febs.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1432-1033.1976.tb11132.x
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https://doi.org/10.1111/j.1432-1033.1976.tb11132.x
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Zitation

Berthold, V., & Geider, K. (1976). Interaction of DNA with DNA‐binding proteins: the characterization of protein HD from Escherichia coli and its nucleic acid complexes. European Journal of Biochemistry, 71(2), 443-449. doi:10.1111/j.1432-1033.1976.tb11132.x.

Zitierlink: https://hdl.handle.net/21.11116/0000-0002-AE02-8

Zusammenfassung

DNA‐binding protein HD with a monomer molecular weight of 9000 was isolated from Escherichia coli cells. The protein occurs as a tetramer under native conditions and binds to single and double‐stranded DNA and also to RNA. DNA complexed with protein HD is a poor template for DNA synthesis by E. coli polymerase I, II or III holoenzyme. Exonuclease III is hindered in degrading HD‐protein‐covered double‐stranded DNA, whereas exonuclease I can digest complexed single‐stranded DNA. Transcription is slightly stimulated in the presence of protein HD.