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Book Chapter

Superresolution fluorescence imaging of mutant Huntingtin aggregation in cells.


Sahl,  S. J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Sahl, S. J., & Vonk, W. I. M. (2019). Superresolution fluorescence imaging of mutant Huntingtin aggregation in cells. In C. M. Gomes (Ed.), Protein Misfolding Diseases. New York: Humana Press. doi:10.1007/978-1-4939-8820-4_15.

Cite as: https://hdl.handle.net/21.11116/0000-0002-B386-C
Fluorescence-based nanoscopy methods (also known as "superresolution" microscopy) have substantially expanded our options to examine the distributions of molecules inside cells with nanometer-scale resolution and molecular specificity. In the biophysical analysis of aggregation-prone misfolded proteins and peptides, this has enabled the visualization of distinct populations of aggregated species such as fibrillar assemblies within intact neuronal cells, well below previous limits of sensitivity and resolution. With the Huntington's disease protein, polyglutamine-expanded mutant huntingtin, as an example, we provide sample preparation and imaging protocols for superresolution microscopy down to the ~30 nm-level.