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Journal Article

Triarylmethane fluorophores resistant to oxidative photobluing.

MPS-Authors
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Butkevich,  A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Bossi,  M. L.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Lukinavicius,  G.
Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Fulltext (public)

3016277.pdf
(Publisher version), 6MB

Supplementary Material (public)

3016277_Suppl_1.pdf
(Supplementary material), 10MB

3016277_Suppl_2.pdf
(Supplementary material), 7MB

3016277_Correction.pdf
(Supplementary material), 282KB

Citation

Butkevich, A., Bossi, M. L., Lukinavicius, G., & Hell, S. W. (2019). Triarylmethane fluorophores resistant to oxidative photobluing. Journal of the American Chemical Society, 141(2), 981-989. doi:10.1021/jacs.8b11036.


Cite as: https://hdl.handle.net/21.11116/0000-0002-B3BD-F
Abstract
Spectral stability of small-molecule fluorescent probes is required for correct interpretation and reproducibility of multicolor fluorescence imaging data, in particular under high (de)excitation light intensities of super-resolution imaging or in single-molecule applications. We propose a synthetic approach to a series of spectrally stable rhodamine fluorophores based on sequential Ru- and Cu-catalyzed transformations, evaluate their stability against photobleaching and photoconversion in context of other fluorophores using chemometric analysis, and demonstrate chemical reactivity of fluorophore photoproducts. The substitution patterns providing the photoconversion-resistant triarylmethane fluorophores have been identified, and the applicability of non-bluing labels in live-cell STED nanoscopy is demonstrated.