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A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning

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Konthur,  Zoltán
Zoltán Konthur, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Citation

Chiew Wen Ch’ng, A., Ahmad, A., Konthur, Z., & Lim, T. S. (2019). A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning. Methods in Molecular Biology, 1904, 377-400. doi:10.1007/978-1-4939-8958-4_18.


Cite as: http://hdl.handle.net/21.11116/0000-0002-BA72-C
Abstract
Panning is a common process used for antibody selection from phage antibody libraries. There are several methods developed for a similar purpose, namely streptavidin mass spectrometry immunoassay (MSIA™) Disposable Automation Research Tips, magnetic beads, polystyrene immunotubes, and microtiter plate. The advantage of using a magnetic particle processor system is the ability to carry out phage display panning against multiple target antigens simultaneously in parallel. The system carries out the panning procedure using magnetic nanoparticles in microtiter plates. The entire incubation, wash, and elution process is then automated in this setup. The system also allows customization for the introduction of different panning stringencies. The nature of the biopanning process coupled with the limitation of the system means that minimal human intervention is required for the infection and phage packaging stage. However, the process still allows for rapid and reproducible antibody generation to be carried out.