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Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry

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Pagel,  Kevin
Institut für Chemie und Biochemie, Freie Universität Berlin;
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Citation

Urner, L. H., Maier, Y. B., Haag, R., & Pagel, K. (2019). Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 30(1), 174-180. doi:10.1007/s13361-018-2063-2.


Cite as: http://hdl.handle.net/21.11116/0000-0002-B5B4-6
Abstract
The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In this work, the gas-phase properties of oligoglycerol detergents (OGDs) are optimized by fine tuning of their molecular structure. Furthermore, a tandem mass spectrometry (MS/MS) approach is presented that estimates the gas-phase properties of detergents simply by studying the dissociation behaviour of protein-detergent complexes (PDCs) formed with the soluble protein β-lactoglobulin (BLG).