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Journal Article

Structure of transcribing RNA polymerase II-nucleosome complex.

MPS-Authors
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Farnung,  L.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Vos,  S. M.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Cramer,  P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Fulltext (public)

3016729.pdf
(Publisher version), 2MB

Supplementary Material (public)

3016729_Suppl_1.pdf
(Supplementary material), 11MB

3016729_Suppl_2.pdf
(Supplementary material), 87KB

3016729_Suppl_3.mov
(Supplementary material), 17MB

3016729_Suppl_4.pdf
(Supplementary material), 2MB

Citation

Farnung, L., Vos, S. M., & Cramer, P. (2018). Structure of transcribing RNA polymerase II-nucleosome complex. Nature Communications, 9: 5432. doi:10.1038/s41467-018-07870-y.


Cite as: https://hdl.handle.net/21.11116/0000-0002-B72C-F
Abstract
Transcription of eukaryotic protein-coding genes requires passage of RNA polymerase II (Pol II) through nucleosomes, but it is unclear how this is achieved. Here we report the cryo-EM structure of transcribing Saccharomyces cerevisiae Pol II engaged with a downstream nucleosome core particle at an overall resolution of 4.4 Å. Pol II and the nucleosome are observed in a defined relative orientation that is not predicted. Pol II contacts both sides of the nucleosome dyad using its clamp head and lobe domains. Structural comparisons reveal that the elongation factors TFIIS, DSIF, NELF, SPT6, and PAF1 complex can be accommodated on the Pol II surface in the presence of the oriented nucleosome. Our results provide a starting point for analysing the mechanisms of chromatin transcription.