Dynamic and non-contact 3D sample rotation for microscopy

Berndt, Frederic; Shah, Gopi; Power, Rory M.; Brugués, Jan; Huisken, Jan

doi:10.1038/s41467-018-07504-3

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Dynamic and non-contact 3D sample rotation for microscopy

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Brugués, Jan
Max Planck Institute for the Physics of Complex Systems, Max Planck Society;

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https://www.nature.com/articles/s41467-018-07504-3
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Berndt, F., Shah, G., Power, R. M., Brugués, J., & Huisken, J. (2018). Dynamic and non-contact 3D sample rotation for microscopy. Nature Communications, 9: 5025. doi:10.1038/s41467-018-07504-3.

Cite as: https://hdl.handle.net/21.11116/0000-0002-BA79-5

Abstract

Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.