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Interferometric Scattering (iSCAT) Microscopy & Related Techniques

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Taylor,  Richard W.
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Sandoghdar,  Vahid
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

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Citation

Taylor, R. W., & Sandoghdar, V. (2019). Interferometric Scattering (iSCAT) Microscopy & Related Techniques. In V. Astratov (Ed.), Label-Free Super-Resolution Microscopy (pp. 25-65). Springer International Publishing.


Cite as: https://hdl.handle.net/21.11116/0000-0002-B9D7-B
Abstract
Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive detection and imaging of nanoparticles to high spatiotemporal resolution. As it was born out of detection principles central to conventional microscopy, we begin by surveying the historical development of the microscope to examine how the exciting possibility for interferometric scattering microscopy with sensitivities sufficient to observe single molecules has become a reality. We discuss the theory of interferometric detection and also issues relevant to achieving a high detection sensitivity and speed. A showcase of numerous applications and avenues of novel research across various disciplines that iSCAT microscopy has opened up is also presented.