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ATP-dependent force generation and membrane scission by ESCRT-III and Vps4

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Schöneberg,  Johannes
Department of Molecular and Cell Biology, University of California, Berkeley, USA;
California Institute for Quantitative Biosciences, University of California, Berkeley, USA;
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;

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Bahrami,  Amir Houshang
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;

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Hummer,  Gerhard
Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society;
Institute of Biophysics, Goethe University, Frankfurt / Main, Germany;

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Citation

Schöneberg, J., Pavlin, M. R., Yan, S., Righini, M., Lee, I.-H., Carlson, L.-A., et al. (2018). ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science, 362(6421), 1423-1428. doi:10.1126/science.aat1839.


Cite as: http://hdl.handle.net/21.11116/0000-0002-D2A0-B
Abstract
The endosomal sorting complexes required for transport (ESCRTs) catalyze reverse-topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated ESCRT-III subunits Snf7, Vps24, and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled. Upon ATP release by photo-uncaging, this system generated forces within the nanotubes that led to membrane scission in a manner dependent upon Vps4 catalytic activity and Vps4 coupling to the ESCRT-III proteins. Imaging of scission revealed Snf7 and Vps4 puncta within nanotubes whose presence followed ATP release, correlated with force generation and nanotube constriction, and preceded scission. These observations directly verify long-standing predictions that ATP-hydrolyzing assemblies of ESCRT-III and Vps4 sever membranes.