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Interaction of DNA with DNA binding proteins III. Infectivity of protein-complexed phage fd DNA in Escherichia coli spheroplasts

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Uhlmann,  A.
Max Planck Institute for Medical Research, Max Planck Society;

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Geider,  Klaus
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Uhlmann, A., & Geider, K. (1977). Interaction of DNA with DNA binding proteins III. Infectivity of protein-complexed phage fd DNA in Escherichia coli spheroplasts. Biochimica et Biophysica Acta: BBA, 474(4), 639-645. doi:10.1016/0005-2787(77)90083-1.


Cite as: https://hdl.handle.net/21.11116/0000-0002-DA67-5
Abstract
Complex formation of circular, single-stranded phage fd DNA with Escherichia coli DNA binding protein HD or phage fd gene 5 protein keeps infection of E. coli spheroplasts at the level of free phage DNA, whereas complexes of this DNA with E. coli DNA unwinding protein show a strongly reduced efficiency of transfection. Displacement of the unwinding protein by HD protein or gene 5 protein also maintains the poor adsorption of the complexes to spheroplasts. Free E. coli DNA unwinding protein and residual amounts of this protein bound to the DNA may interfere with the adsorption and the uptake of the phage genome.