Interaction of DNA with DNA binding proteins II. Displacement of Escherichia 
coli DNA unwinding protein and the condensed structure of DNA complexed with 
protein HD

Zentgraf , Hanswalter; Berthold, Verena; Geider, Klaus

doi:10.1016/0005-2787(77)90082-X

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Interaction of DNA with DNA binding proteins II. Displacement of Escherichia coli DNA unwinding protein and the condensed structure of DNA complexed with protein HD

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Berthold, Verena
Max Planck Institute for Medical Research, Max Planck Society;

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Geider, Klaus
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/000527877790082X/pdf?md5=182f141902bac626176700ae107d291b&pid=1-s2.0-000527877790082X-main.pdf
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Citation

Zentgraf, H., Berthold, V., & Geider, K. (1977). Interaction of DNA with DNA binding proteins II. Displacement of Escherichia coli DNA unwinding protein and the condensed structure of DNA complexed with protein HD. Biochimica et Biophysica Acta: BBA, 474(4), 629-638. doi:10.1016/0005-2787(77)90082-X.

Cite as: https://hdl.handle.net/21.11116/0000-0002-DEC6-5

Abstract

Three DNA binding proteins from Escherichia coli cells have been complexed with single-stranded phage fd DNA. Electron microscopy reveals granular substructures in the complexes formed with protein HD. In complexes of DNA unwinding protein with fd DNA both protein HD and phage-coded gene 5 protein partially displace the unwinding protein which results in the formation of structures characteristic for the DNA complexes formed with either protein HD or gene 5 protein alone. Combination of protein HD with double-stranded phage T7 DNA leads to a progressive folding and condensing of the genome. The structures observed are discussed in relation to current concepts of the packing of DNA in protein complexes.