English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Competition between oxalate and phosphate during active calcium accumulation by sarcoplasmic vesicles

MPS-Authors
/persons/resource/persons229690

Beil,  Frank U.
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons229692

von Chak,  Dorothea
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons93324

Hasselbach,  Wilhelm
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons204155

Weber,  Hans Hermann
Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.degruyter.com/downloadpdf/j/znc.1977.32.issue-3-4/znc-1977-3-421/znc-1977-3-421.xml
(Any fulltext)

https://doi.org/10.1515/znc-1977-3-421
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Beil, F. U., von Chak, D., Hasselbach, W., & Weber, H. H. (1977). Competition between oxalate and phosphate during active calcium accumulation by sarcoplasmic vesicles. Zeitschrift für Naturforschung, C: Journal of Biosciences, 32(3-4), 281-287. doi:10.1515/znc-1977-3-421.


Cite as: https://hdl.handle.net/21.11116/0000-0002-E4C9-A
Abstract
Sarcoplasmic Calcium Uptake, Oxalate and Phosphate Uptake, Oxalate-Phosphate Competition, Calcium Turnover

1. During ATP supported active calcium uptake oxalate as well as phosphate are accumulated with calcium. The uptake of calcium exceeds that of both anions by a small quantity - accounting for calcium binding to vesicular proteins and lipids.

2. From assay media containing phosphate and oxalate - nearly exclusively either oxalate or phosphate are taken up together with calcium by the sarcoplasmic reticulum vesicles. The mutual exclusion occurs in a very narrow concentration range of the anions.

3. In solutions containing phosphate and oxalate, calcium phosphate or calcium oxalate pre­ cipitates are formed according to their solubility properties.

4. When phosphate prevents oxalate from being taken up, calcium transport is inhibited. In­ hibition occurs, because the concentration of ionized calcium inside the vesicles rises approximately 100-fold when oxalate is replaced by phosphate. The activity of the calcium dependent ATPase parallels the calcium uptake activity.

5. It is excluded that the inhibition of calcium uptake produced by phosphate is caused by an enhanced permeability of the sarcoplasmic reticulum membranes for calcium in the presence of phosphate.