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Journal Article

A chemogenetic approach for the optical monitoring of voltage in neurons

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Sundukova, M., Prifti, E., Bucci, A., Kirillova, K., Serrao, J., Reymond, L., et al. (2019). A chemogenetic approach for the optical monitoring of voltage in neurons. Angewandte Chemie International Edition, 58(8), 2341-2344. doi:10.1002/anie.201812967.


Cite as: http://hdl.handle.net/21.11116/0000-0002-E506-5
Abstract
Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons.