Fluorescence protein complementation in microscopy: applications beyone 
detacting bi-molecular interactions

Avilov, Sergiy; Aleksandrova, Nataliia

doi:10.1088/2050-6120/aaef01

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Fluorescence protein complementation in microscopy: applications beyone detacting bi-molecular interactions

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Avilov, Sergiy
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Avilov, S., & Aleksandrova, N. (2019). Fluorescence protein complementation in microscopy: applications beyone detacting bi-molecular interactions. Methods in Applied Fluorescence, 7, 012001. doi:10.1088/2050-6120/aaef01.

Cite as: https://hdl.handle.net/21.11116/0000-0003-6E19-7

Abstract

Conventional fragments of fluorescent proteins used in bimolecular fluorescence complementation technique (BiFC), form light-emitting species only when they are kept in close proximity by interacting proteins of interest. By contrast, certain fluorescent protein fragments complement spontaneously, namely those corresponding to the 1st to 10th beta-strands (GFP1-10) and the 11th beta-strand of superfolder GFP (GFP11). They were designed as folding reporters for high throughput expression and structure biology. Besides, for light microscopy, self-associating fluorescent protein fragments constitute a valuable and sometimes unique tool. The GFP11 tag is very advantageous when a full-length fluorescent protein cannot be fused to a protein of interest, namely for live imaging of certain pathogens. Self-associating GFP fragments enable live labelling of specific synapses, visualization of proteins topology and their exposure to particular subcellular compartments. Present review aims to attract attention of scientific community to these tools and to inspire their further development and applications.