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Journal Article

Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta

MPS-Authors
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Fandino,  Richard A.
Research Group Dr. M. Knaden, Insect Behavior, Department of Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Haverkamp,  Alexander
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

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Bisch-Knaden,  Sonja
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Zhang,  Jin
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Bucks,  Sascha
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Nguyen Thi,  Tu Anh
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Rybak,  Jürgen
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Knaden,  Markus
Research Group Dr. M. Knaden, Insect Behavior, Department of Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Hansson,  Bill S.
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

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Grosse-Wilde,  Ewald
Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society;

External Resource

http://dx.doi.org/10.1073/pnas.1902089116
(Publisher version)

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Fulltext (public)

HAN321.pdf
(Publisher version), 2MB

Supplementary Material (public)

HAN321s1.zip
(Supplementary material), 87MB

Citation

Fandino, R. A., Haverkamp, A., Bisch-Knaden, S., Zhang, J., Bucks, S., Nguyen Thi, T. A., et al. (2019). Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta. Proceedings of the National Academy of Sciences of the United States of America, 116(31), 15677-15685. doi:10.1073/pnas.1902089116.


Cite as: https://hdl.handle.net/21.11116/0000-0002-F622-2
Abstract
The hawkmoth Manduca sexta and one of its preferred hosts in the North American Southwest, Datura wrightii, share a model
insect–plant relationship based on mutualistic and antagonistic
life-history traits. D. wrightii is the innately preferred nectar source
and oviposition host for M. sexta. Hence, the hawkmoth is an important
pollinator while the M. sexta larvae are specialized herbivores
of the plant. Olfactory detection of plant volatiles plays a
crucial role in the behavior of the hawkmoth. In vivo, the odorant
receptor coreceptor (Orco) is an obligatory component for the function
of odorant receptors (ORs), a major receptor family involved in
insect olfaction. We used CRISPR-Cas9 targeted mutagenesis to
knock out (KO) the MsexOrco gene to test the consequences of a
loss of OR-mediated olfaction in an insect–plant relationship. Neurophysiological
characterization revealed severely reduced antennal
and antennal lobe responses to representative odorants emitted by
D. wrightii. In a wind-tunnel settingwith a flowering plant, Orco KO
hawkmoths showed disrupted flight orientation and an ablated
proboscis extension response to the natural stimulus. The Orco KO
gravid female displayed reduced attraction toward a nonflowering
plant. However, more than half of hawkmoths were able to use
characteristic odor-directed flight orientation and oviposit on the
host plant. Overall, OR-mediated olfaction is essential for foraging
and pollination behaviors, but plant-seeking and oviposition behaviors are sustained through additional OR-independent sensory cues.