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Journal Article

Osmolytes modulate polyglutamine aggregation in a sequence dependent manner

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Saha,  Itika
Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Saha, I., Singh, V., Burra, G., & Thakur, A. K. (2018). Osmolytes modulate polyglutamine aggregation in a sequence dependent manner. Journal of Peptide Science, 24(8-9): UNSP e3115. doi:10.1002/psc.3115.


Cite as: https://hdl.handle.net/21.11116/0000-0003-10EE-F
Abstract
Osmolytes stabilize protein structure and suppress protein aggregation. The mechanism of how osmolytes impact polyglutamine (polyQ) aggregation implicated in Huntington's disease was studied. By using a reverse‐phase chromatography assay, we show that methylamines‐trimethylamine N‐oxide and betaine are generic in enhancing polyQ aggregation, while a disaccharide trehalose and an amino acid citrulline moderately retard polyQ aggregation in a sequence specific manner. Despite the altered kinetics, the fundamental nucleation mechanism of polyQ aggregation and the nature of end stage aggregates remains unaffected. These results highlight the importance of using osmolytes as modulatory agents of polyQ aggregation.