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Journal Article

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

MPS-Authors
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Ramm,  Beatrice
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Glock,  Philipp
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Schwille,  Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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https://www.jove.com/video/58139/
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Citation

Ramm, B., Glock, P., & Schwille, P. (2018). In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers. Journal of Visualized Experiments, (137): e58139. doi:10.3791/58139.


Cite as: https://hdl.handle.net/21.11116/0000-0003-27DF-7
Abstract
We provide a protocol for in vitro self-organization assays of MinD and MinE on a supported lipid bilayer in an open chamber. Additionally, we describe how to enclose the assay in lipid-clad PDMS microcompartments to mimic in vivo conditions by reaction confinement.