In Vivo Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression 
of Spider Silk-Inspired Peptides

Mickoleit, Frank; Borkner, Christian B.; Toro-Nahuelpan, Mauricio; Herold, Heike M.; Maier, Denis S.; Plitzko, Jürgen M.; Scheibel, Thomas; Schueler, Dirk

doi:10.1021/acs.biomac.7b01749

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In Vivo Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides

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Toro-Nahuelpan, Mauricio
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Plitzko, Jürgen M.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Mickoleit, F., Borkner, C. B., Toro-Nahuelpan, M., Herold, H. M., Maier, D. S., Plitzko, J. M., et al. (2018). In Vivo Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides. Biomacromolecules, 19(3), 962-972. doi:10.1021/acs.biomac.7b01749.

Cite as: https://hdl.handle.net/21.11116/0000-0003-131A-B

Abstract

Magnetosomes are natural magnetic nanoparticles with exceptional properties that are synthesized in magnetotactic bacteria by a highly regulated biomineralization process. Their usability in many applications could be further improved by encapsulation in biocompatible polymers. In this study, we explored the production of spider silk-inspired peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion of different silk sequence-like variants to abundant magnetosome membrane proteins enhanced magnetite biomineralization and caused the formation of a proteinaceous capsule, which increased the colloidal stability of isolated particles. Furthermore, we show that spider silk peptides fused to a magnetosome membrane protein can be used as seeds for silk fibril growth on the magnetosome surface. In summary, we demonstrate that the combination of two different biogenic materials generates a genetically encoded hybrid composite with engineerable new properties and enhanced potential for various applications.