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Alpha- and beta-mannan utilization by marine Bacteroidetes

MPS-Authors

Chen,  Jing
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Robb,  Craig
University Bremen - MPI Joint Research Group for Marine Glycobiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Kappelmann,  Lennart
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Harder,  Jens
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Avci,  Burak
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Amann,  Rudolf I.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Hehemann,  Jan Hendrik
University Bremen - MPI Joint Research Group for Marine Glycobiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Teeling,  Hanno
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Chen, J., Robb, C., Unfried, F., Kappelmann, L., Markert, S., Song, T., et al. (2018). Alpha- and beta-mannan utilization by marine Bacteroidetes. Environmental Microbiology, 20(11), 4127-4140. doi:10.1111/1462-2920.14414.


Cite as: http://hdl.handle.net/21.11116/0000-0003-B81F-C
Abstract
Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.