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Journal Article

Laminarin Quantification in Microalgae with Enzymes from Marine Microbes

MPS-Authors
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Becker,  Stefan
HGF MPG Joint Research Group for Deep Sea Ecology & Technology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Hehemann,  Jan Hendrik
University Bremen - MPI Joint Research Group for Marine Glycobiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Becker_2018_01.pdf
(Publisher version), 564KB

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Citation

Becker, S., & Hehemann, J. H. (2018). Laminarin Quantification in Microalgae with Enzymes from Marine Microbes. BIO-PROTOCOL, 8(8): e2666. doi:10.21769/BioProtoc.2666.


Cite as: http://hdl.handle.net/21.11116/0000-0003-B7D2-1
Abstract
The marine beta-glucan laminarin is an abundant storage polysaccharide in microalgae. High production rates and rapid digestion by heterotrophic bacteria turn laminarin into an ideal carbon and energy source, and it is therefore a key player in the marine carbon cycle. As a main storage glucan laminarin also plays a central role in the energy metabolism of the microalgae (Percival and Ross, 1951; Myklestad, 1974; Painter, 1983). We take advantage of enzymes that digest laminarin selectively and can thereby quantify only this polysaccharide in environmental samples. These enzymes hydrolyze laminarin into glucose and oligosaccharides, which are measured with a standard reducing sugar assay to obtain the laminarin concentration. Prior to this assay, the three enzymes need to be produced via heterologous expression and purification. The assay can be used to monitor laminarin concentrations in environmental microalgae, which were concentrated from seawater by filtering, or in samples derived from algal lab cultures.