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Journal Article

Temporal and reversible control of a DNAzyme by orthogonal photoswitching

MPS-Authors

Valero,  Julian
Max Planck Fellow Chemical Biology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

Famulok,  Michael
Max Planck Fellow Chemical Biology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Citation

Haydell, M. W., Centola, M., Adam, V., Valero, J., & Famulok, M. (2018). Temporal and reversible control of a DNAzyme by orthogonal photoswitching. Journal of the American Chemical Society, 140(49), 16868-16872. doi:10.1021/jacs.8b08738.


Cite as: http://hdl.handle.net/21.11116/0000-0003-53E3-F
Abstract
The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzyme. We show that its catalytic function can be turned on and off reversibly upon irradiation with specific wavelengths of light. The system responds orthogonally to a selection of irradiation wavelengths and durations of irradiation. Furthermore, the DNAzyme exhibits reversible switching and retains this ability throughout multiple switching cycles. We apply our system as a light-controlled 4:2 multiplexer. Orthogonally photoswitchable DNAzyme-based catalysts as introduced here have potential use for controlling complex logical operations and for future applications in DNA nanodevices.